This study investigates the effect of different fibers (hooked steel, recycled steel, macrosynthetic and ladder glass) on the mechanical behavior and impact resistance of concrete segments. Given the challenges of dynamic and impact loads during the installation and operation of tunnels, the use of fiber concrete as an alternative to conventional reinforced concrete has been considered to improve structural performance and reduce costs. In this study, concrete samples with different fiber volume percentages (1.5%, 2% and 2.5%) were made and subjected to compressive, tensile, flexural and impact strength tests. The results showed that all types of fibers used significantly improved the mechanical properties of concrete. In particular, recycled steel fibers had the greatest effect in increasing compressive, tensile and flexural strengths. This sample with 2.5% fibers showed an increase in compressive strength of 36.74%, tensile strength of 42.57%, and flexural strength of 44.80% in the 28-day sample, which was more than the control sample. While macrosynthetic fibers showed the best performance in energy absorption and impact resistance, it was also observed that the addition of fibers significantly reduced the width of cracks and changed the behavior of concrete from brittle to semi-ductile. So that macrosynthetic fibers with 2.5% fibers had a 3 mm crack, while the control sample completely disintegrated under impact. This not only increased the energy absorption capacity, but also improved the durability and stability of the structure.